Mass spectrometry in forensic analysis is a technology provides a powerful tool for forensic studies. For forensic researchers investigating their samples for unknown compounds, drug metabolites, chemicals or hazards, novel psychoactive substances that have never been previously detected or characterized. The role of mass spectroscopy in forensic science may be characterized as either molecular or elemental analysis. comparatively little volatile nonionic molecules found in a very sort of forensic samples may be analyzed with electron and chemical ionization of single-stage mass analyzers that give relative molecular mass and structural data. Non-polar and polar molecules and their metabolites habitually found in biological matrices along with alternative analytes of forensic interest may be determined by using electrospray.